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Protective Effect of Resveratrol on Biomarkers of Oxidative Stress Induced by Iron/Ascorbate in Mouse Spermatozoa

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dc.contributor.author Izquierdo Vega, Jeannett Alejandra
dc.date.accessioned 2016-11-03T22:46:13Z
dc.date.available 2016-11-03T22:46:13Z
dc.date.issued 2014
dc.identifier.citation María Angélica Mojica-Villegas, Jeannett Alejandra Izquierdo-Vega, Germán Chamorro-Cevallos and Manuel Sánchez-Gutiérrez. (2014). Protective Effect of Resveratrol on Biomarkers of Oxidative Stress Induced by Iron/Ascorbate in Mouse Spermatozoa. Nutrients. 6: 489-503. es
dc.identifier.uri https://repository.uaeh.edu.mx/bitstream/123456789/17323
dc.description.abstract Resveratrol (RVT) is a polyphenolic compound found mainly in the grape and attributed with various pharmacological properties, among them their antioxidant activity. In the present study, we assess the antioxidant activity of resveratrol on oxidative damage induced by ferrous iron/ascorbate (100 ?M/150 ?M) in sperm of CD1+ mice. We evaluated several parameters in spermatozoa treated with or without resveratrol: (i) sperm quality analysis; (ii) mitochondrial transmembrane potential (??m); (iii) ROS generation; (iv) superoxide dismutase (SOD) activity; (v) glutathione peroxidase (GPX) activity; (vi) lipid peroxidation; (vii) and in vitro fertilization (IVF) capability. Spermatozoa treated with RVT (15 ?g/mL) before ferrous iron/ascorbate treatment exhibited: a significant increase in motility (8-fold), a significant increase in viability (2-fold), a significant increase in ??m (1.15-fold), accompanied with a significant decrease in the generation of ROS (4.96-fold), a significant decrease in GPX activity (1.32-fold), and a significant decrease in lipid peroxidation concentration (10.29-fold) relative to spermatozoa treated with ferrous iron/ascorbate; however, no changes in SOD activity were observed. Finally, spermatozoa treated with RVT before ferrous iron/ascorbate treatment showed a significant increase in oocyte fertilization (1.2-fold), relative to spermatozoa treated with ferrous iron/ascorbate. These results suggest that RVT possesses antioxidant properties that may prevent the deleterious effects produced by oxidative damage on spermatozoa, resulting in the maintenance of fertility. es
dc.language es
dc.subject Toxicología Preclínica es
dc.title Protective Effect of Resveratrol on Biomarkers of Oxidative Stress Induced by Iron/Ascorbate in Mouse Spermatozoa es
dc.type Article


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