Electroacupuncture improves gait locomotion, H-reflex and ventral root potentials of spinal compression injured rats.
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This study explored the effect of electroacupuncture stimulation (EA) on alterations in the Hoffman reflex
(H-reflex) response and gait locomotion provoked by spinal cord injury (SCI) in the rat. A compression
lesion of the spinal cord was evoked by insufflating a Fogarty balloon located in the epidural space at
the T8?9 spinal level of adult Wistar male rats (200?250 gr; n = 60). In different groups of SCI rats, EA
(frequencies: 2, 50 and 100 Hz) was applied simultaneously to Huantiao (GB30), Yinmen (BL37), Jizhong (GV6) and Zhiyang (GV9) acupoints from the third post-injury day until the experimental session. At 1,
2, 3 and 4 post-injury weeks, the BBB scores of the SCI group of rats treated with EA at 50 Hz showed a
gradual but greater enhancement of locomotor activity than the other groups of rats. Unrestrained gait
kinematic analysis of SCI rats treated with EA?50 Hz stimulation showed a significant improvement in
stride duration, length and speed (p < 0.05), whereas a discrete recovery of gait locomotion was observed
in the other groups of animals. After four post-injury weeks, the H-reflex amplitude and H-reflex/M
wave amplitude ratio obtained in SCI rats had a noticeable enhancement (217%) compared to sham rats
(n = 10). Meanwhile, SCI rats treated with EA at 50 Hz manifested a decreased facilitation of the H-reflex
amplitude and H/M amplitude ratio (154%) and a reduced frequency-dependent amplitude depression of
the H-reflex (66%). In addition, 50 Hz-EA treatment induced a recovery of the presynaptic depression of
the Gs-VRP evoked by PBSt conditioning stimulation in the SCI rat (63.2 ± 8.1%; n = 9). In concordance with
the latter, it could be suggested that 50 Hz-EA stimulation reduced the hyper-excitability of motoneurons
and provokes a partial improvement of the locomotive performance and H reflex responses by a possible
recovery of presynaptic mechanisms in the spinal cord of experimentally injured rats.
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Escobar-Corona, C., Torres-Castillo, S., Rodríguez-Torres, E. E., Segura-Alegría, B., Jiménez-Estrada, I., & Quiroz-González, S. (2017). Electroacupuncture improves gait locomotion, H-reflex and ventral root potentials of spinal compression injured rats. Brain Research Bulletin.