Conditioning and characterization of electrotrophic bacteria for biofilm formation

Abstract

The whey is generated during cheesemaking as phase in which are protein milk not precipitated. It Is highly polluting because of its high organic load generates high values of chemical oxygen demand In aquifers. In Mexico, the whey spilling in aquifers is a constant problem. To this end, bioeletrochemical sistems are a viable alternative, because they have low complexity, time reduced operation, low cost and environmentally friendly. In them, bacteria act as donors or electron acceptor adhere to a biocompatible, solid and conductor material forming a biofilm, this arrangement is known as microbial electrode. Therefore, in this work the conditioning and characterization of electron acceptor bacteria in whey; grown by aerobic and anaerobic conditions, also were dyed by Gram stains. Finally, in samples embedded graphite rods which were analyzed later by electrochemical techniques. Results indicate that the specimen is a facultatively anaerobic bacterial consortium incorporated by five Gram-negative and one Gram-positive constituents. They can wrong in extract broth peptone, agares MacConkey and eosin-methylene blue with different growth microscopic and macroscopic, capable of generating microbial electrodes stable in whey.