Evaluación genotóxica de un novedoso nanohíbrido para la liberación de moléculas bioactivas
Resumen
En las últimas dos décadas, los nanomateriales híbridos orgánicos-inorgánicos han alcanzado un desarrollo intensivo. Sin embargo, antes de su posible aplicación, se requiere de una evaluación completa y adecuada tanto de sus propiedades fisicoquímicas y beneficios, como de los potenciales efectos tóxicos para la salud humana. El objetivo de este estudio fue determinar el potencial genotóxico in vivo de nanohíbridos diseñados a base de hidróxidos dobles laminares/ß-hidroxi-ß-metil butirato/colágeno (ZnAl/HMB/Col) tras una exposición oral aguda. El potencial genotóxico fue evaluado en ratas macho Sprague-Dawley (SD) de 9 semanas de edad tras 7 días continuos de exposición oral a los nanohíbridos, a una dosis de 2,000 mg/kg. No se observó una diferencia estadísticamente significativa entre el número de micronúcleos (MN) y la administración de los tratamientos sobre los valores del grupo control.
Descargas
Citas
Casanova, F., Nascimento, L. G. L., Silva, N. F. N., de Carvalho, A. F. y Gaucheron, F. (2021). Interactions between caseins and food-derived bioactive molecules: A review. Food Chemistry, 359. https://doi.org/10.1016/j.foodchem.2021.129820
Chang, C.-H. I., Lee, Y.-H., Liao, Z.-H., Hung-Chih Chen, M., Peng, F.-C. y Lin, J.-J. (2021). Composition of nanoclay supported silver nanoparticles in furtherance of mitigating cytotoxicity and genotoxicity. PLoS ONE, 16(2). https://doi.org/10.1371/journal.pone.0247531
da Silva, A. F., de Pádua, G. S., de Araújo, D. T., Vieira, C. A. y de Faria, E. H. (2020). Immobilization of L-alanine into natural kaolinite via amidation catalyzed by boric acid for the development of biohybrid materials. Journal of Solid State Chemistry, 287. https://doi.org/10.1016/j.jssc.2020.121332
Daud, M., Hai, A., Banat, F., Wazir, M. B., Habib, M., Bharath, G. y Al-harthi, M. A. (2019). A review on the recent advances , challenges and future aspect of layered double hydroxides ( LDH ) – Containing hybrids as promising adsorbents for dyes removal. Journal of Molecular Liquids, 288. https://doi.org/10.1016/j.molliq.2019.110989
del Arco, A. M., Fernández, C. Martín, V. R. (2010). Solubility and release of fenbufen intercalated in Mg , Al and Mg , Al , Fe layered double hydroxides ( LDH ): The effect of Eudragit s S 100 covering. Journal of Solid State Chemistry, 183, 3002–3009. https://doi.org/10.1016/j.jssc.2010.10.017
Fernandes, F.M., Baradari, C. S. (2014). Integrative strategies to hybrid lamellar compounds: an integration challenge. Applied Clay Science, 100. https://doi.org/doi.org/10.1016/j.clay.2014.05.013
García-Rodríguez, A., Kazantseva, L., Vila, L., Rubio, L., Velázquez, A., Ramírez, M. J., Marcos, R. y Hernández, A. (2019). Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials. Nanomaterials, 9(12). https://doi.org/10.3390/nano9121677
Giray Bugra, A. y Türkez, H. (2018). Investigation of the Genotoxicity of Aluminum Oxide, 3-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro. International Journal of Toxicology, 37(3), 216–222. https://doi.org/10.1177/1091581818775709
Gu, Z., Yan, S., Cheong, S., Cao, Z., Zuo, H., Anita, C., Rolfe, B. E. y Xu, Z. P. (2017). Layered double hydroxide nanoparticles : Impact on vascular cells , blood cells and the complement system. Colloid and Interface Science. https://doi.org/10.1016/j.jcis.2017.10.069
Kim, D. U., Chung, H. C., Choi, J., Sakai, Y. y Lee, B. Y. (2018). Oral intake of low-molecular-weight collagen peptide improves hydration, elasticity, and wrinkling in human skin: A randomized, double-blind, placebo-controlled study. Nutrients, 10(7). https://doi.org/10.3390/nu10070826
Kura, A. U., Hussein, M. Z., Fakurazi, S. y Arulselvan, P. (2014). Layered double hydroxide nanocomposite for drug delivery systems ; bio-distribution , toxicity and drug activity enhancement. Chemistry Central Journal, 8(47). https://doi.org/https://doi.org/10.1186/s13065-014-0047-2
Kura, A. U., Saifullah, B., Cheah, P. S., Hussein, M. Z., Azmi, N. y Fakurazi, S. (2015). Acute oral toxicity and biodistribution study of zinc-aluminium-levodopa nanocomposite. Nanoscale Research Letters, 10(1). https://doi.org/10.1186/s11671-015-0742-5
Maisanaba, S., Pichardo, S., Puerto, M., Gutiérrez-Praena, D., Cameán, A. M. y Jos, A. (2015). Toxicological evaluation of clay minerals and derived nanocomposites: A review. Environmental Research, 138, 233–254. https://doi.org/10.1016/J.ENVRES.2014.12.024
Majumder, J. y Minko, T. (2021). Multifunctional and stimuli-responsive nanocarriers for targeted therapeutic delivery. Expert Opinion on Drug Delivery, 18(2), 205–227. https://doi.org/10.1080/17425247.2021.1828339
Malaviya, P., Shukal, D. y Vasavada, A. R. (2020). Nanotechnology-based Drug Delivery, Metabolism and Toxicity. Current Drug Metabolism, 20(14). https://doi.org/10.2174/1389200221666200103091753
OECD. (2016). OECD TG474: Mammalian Erythrocyte Micronucleus Test. In Organisation for Economic Co-operation and Development publishing.
Oh, Y. S. (2016). Bioactive compounds and their neuroprotective effects in diabetic complications. Nutrients, 8(8). https://doi.org/10.3390/nu8080472
Ojha, B., Jain, V. K., Mehra, N. K. y Jain, K. (2021). Nanotechnology: Introduction and Basic Concepts. Dendrimers in Nanomedicine. https://doi.org/10.1201/9781003029915-1
Shi, J., Votruba, A. R., Farokhzad, O. C. y Langer, R. (2010). Nanotechnology in drug delivery and tissue engineering: From discovery to applications. Nano Letters, 10(9). https://doi.org/10.1021/nl102184c
Silion, M., Hritcu, D., Lisa, G. y Popa, M. I. (2012). New hybrid materials based on layered double hydroxides and antioxidant compounds. Preparation, characterization and release kinetic studies. Journal of Porous Materials, 19(3), 267–276. https://doi.org/10.1007/s10934-011-9473-x
Sultana, N., Bora, P. y Sarma, B. (2019). Nanocarriers in drug delivery system: Eminence and confront. Smart Nanocontainers: Micro and Nano Technologies. https://doi.org/10.1016/B978-0-12-816770-0.00010-1
Yonamine, C. Y., Gerlinger-Romero, F., Guimara, L., Barrera, R., Maria, S., Nunes, T., Guimarães-Ferreira, L., Yonamine, C. Y., Salgueiro, R. B. y Nunes, M. T. (2017). Effects of beta-hydroxy-beta-methylbutyrate (HMB) on the expression of ubiquitin ligases, protein synthesis pathways and contractile function in extensor digitorum longus (EDL) of fed and fasting rats. Journal of Physiological Sciences, 68(2), 165–174. https://doi.org/10.1007/s12576-016-0520-x
