Optimization of the diaphonization and staining technique of Piovesana (2014), applied to the fish Gymnocorymbus ternetzi
Abstract
The diaphonization technique consists in making the soft tissues of an organism transparent in order to observe the bone and cartilaginous tissues. Specific dyes are used for these purposes, mainly red alizarin for bone. There are no standardized protocols for each species, since it is generalized for large groups, being the group of fishes one of the least defined. In this article a protocol of the technique was developed for the nun fish (Gymnocorymbus ternetzi), there are included, the necessary chemicals, and the costs in Mexican pesos.
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References
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