Cryopreservation of Laelia speciosa in danger of extinction
Abstract
Among the diversity that Mexico possesses are orchids, 44 percent of which are endemic. Germplasm banks are alternative for the rescue and preservation of innumerable resources. The objective of this work was to cryopreserve Laelia speciosa using encapsulation-dehydration and vitrification techniques. Micropropagation was in Murashige and Skoog medium with malt extract (500 mg·L-1). Encapsulation consisted of protocorms with sodium alginate, 0.75 M calcium chloride, 0.75 M pretreatment for 24 hours and exposed to silica gel for 5 hours, obtaining of 80 to 100 percent survival and regeneration. For the vitrification protocol, the protocorms were pre-cultured in 0.3 M sucrose for 3 days, 20 min in loading treatment and dehydration with PVS2 for 60 min, obtaining 100 percent survival and regeneration. Therefore, protocols for long-term preservation of Laelia speciosa were established.
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