Application of scattered light intensity patterns in biomass estimation
Abstract
This paper presents a technique for biomass estimation in bacterial culture of biotechnological interest, by means of scattered light intensity patterns. For this work a system was implemented to perform the acquisition of light scattered patterns, and polynomial regression and support vector regression techniques were applied to estimate concentration values. The effectiveness of this technique was evaluated using a spectrophotometer as a reference instrument. The results obtained allow us to observe similarities in the behavior of the parameters of the scattering pattern in comparison with the transmittance and absorbance measured with a spectrophotometer. The regression models derived from regressions analysis allow to compensate the accuracy limitations of the scattered light intensity pattern acquisition system.
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References
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