Relaxant effect of thymol on smooth muscle

Keywords: Thymol, medicinal plant, spasmolytic, smooth muscle


Thymol is a phenolic monoterpene with colorless crystalline shapes or white crystalline powder with a spicy, caucasian and sweet taste. Its chemical structure is characterized by a hydroxyl group (-OH) attached to a benzene ring. It provides for the hydroxylation of P-cymine along with its carvacrol’s isomer. In this molecule different therapeutic properties have been registered in this molecule, such as spasmolytic, anticancer, anti-inflammatory, fungicidal, antibacterial, antioxidant and hepatoprotective. This monoterpene is found in different species of the genus Thymus, especially T. Vulgaris and T. zigis with a percentage that fluctuates between 10% and 60% found in the aerial part of both plants, likewise, it is also found in Lagoecia cuminoides between a 73% and 95% and in Trachyspermum copticum between 37.2 and 72.3% in the seed and fruits of this plant. Studies on its beneficial effects on health have been carried out in different species, highlighting rats, mice, rabbits, guinea pigs, chickens, human cell lines; distinguishing human studies have been conducted 3 studies to decrease the effects of asthma, antitussive and anti-inflammatory effect on the lungs. The aim of this narrative article is to describe the processes of contraction and relaxation of smooth muscle and the spasmolytic effect of thymol in different smooth muscle tissues in rats, mice, guinea pigs and rabbits. Through this research, it is suggested to the scientific community to study the possible mechanisms of action of this molecule in the different types of smooth muscle tissue for subsequent clinical studies


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Gao T, Zhou H, Zhou W, Hu L, Chen J, Shi Z. The fungicidal activity of thymol against Fusarium graminearum via inducing lipid peroxidation and disrupting ergosterol biosynthesis. Molecules. 2016; 21(6):770.

Gholami-Ahangaran M, Ahmadi-Dastgerdi A, Azizi S, Basiratpour A, Zokaei M, Derakhshan M. Thymol and carvacrol supplementation in poultry health and performance. Vet. Med. Sci. 2022;8(1):267-88.

Salehi B, Mishra AP, Shukla I, Sharifi-Rad M, Contreras MDM, Segura-Carretero A, et al. Thymol, thyme, and other plant sources: Health and potential uses. Phytother. Res. 2018;32(9):1688-706.

Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int. J. Food. Microbiol. 2004;94(3):223-53.

Baser KHC, Tumen G. Composition of the essential oil of Lagoecia cuminoides L. from Turkey. J. Essent. Oil. Res. 1994;6(5):545-6.

Rasooli I, Fakoor MH, Yadegarinia D, Gachkar L, Allameh A, Rezaei MB. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. Int. J. Food. Microbiol. 2008;122(1-2):135-9.

Nagoor MF, Javed H, Al TH, Azimullah S, Ojha SK. Pharmacological properties, and molecular mechanisms of thymol: prospects for its therapeutic potential and pharmaceutical development. Front. Pharmacol. 2017;8:380.

Schönknecht K, Krauss H, Jambor J, Fal AM. Treatment of cough in respiratory tract infections- the effect of combining the natural active compounds with thymol. Wiad. Lek. 2016;69(6):791-8.

Borugä O, Jianu C, Misca C, Golet I, Gruia AT, Horhat FG. Thymus vulgaris essential oil: chemical composition and antimicrobial activity. J. Med. Life. 2014;7 Spec N. 3 (spec iss 3):56-60.

Teixeira APC, Nóbrega RO, Lima EO, Araújo WO, Lima IO. Antifungal activity study of the monoterpene thymol against Cryptococcus neoformans. Nat. Prod. Res. 2020;34(18):2630-33.

Micucci M, Protti M, Aldini R, Frosini M, Corazza I, Marzetti C, et al. Thymus vulgaris L. Essential oil solid formulation: chemical profile and spasmolytic and antimicrobial effects. Biomolecules. 2020;10(6):860.

Ribeiro ANS, Diniz PB, Pinheiro SM, Albuquerque-Junior RL, Thomazzi SH. Gastroprotective effects of thymol on acute and chronic ulcers in rats: the role of prostaglandins, ATP- sensitive K(+) channels, and gastric mucus secretion. Chem. Biol. Interact. 2016;244:121-8.

Rúa J, Del Valle P, Arriaga D, Fernández-Álvarez L, García-Armesto MR. Combination of carvacrol and thymol: antimicrobial activity against Staphylococcus aureus and antioxidant activity. Foodborne. Pathog. Dis. 2019;16(9):622-29.

Islam MT, Khalipha ABR, Bagchi R, Mondal M, Smrity Sz, Uddin SJ, et. al. Anticancer activity of thymol: a literature-based review and docking study with emphasis on its anticancer mechanisms. IUBMB. Life. 2019;71(1):9-19.

Fachini-Queiroz FC, Kummer R, Estavao-Silva CM, Carvalho MD, Cunha JM, Grespan R, et al. Effects of thymol and carvacrol, constituents of Thymus vulgaris L. essential oil, on the inflammatory response. Evid. Based. Complement. Alternat. Med. 2012;2012:657026.

Khazdair MR, Rajabi O, Balali-Mood M, Beheshti F, Boskabady MH. The effect of Zataria multiflora on pulmonary function tests, hematological and oxidant/antioxidant parameters in sulphur mustard exposed veterans, a randomized doubled-blind clinical trial. Environ. Toxicol. Pharmacol. 2018;58:180-88.

Gavliakova S, Biringerova Z, Buday T, Brozmanova M, Calkovsky V, Poliacek I, et al. Antitussive effects of nasal thymol challenges in healthy volunteers. Respir. Physiol. Neurobiol. 2013;187(1):104-7.

Alavinezhad A, Hedayati M, Boskabady MH. The effect of Zataria multiflora and carvacrol on wheezing, FEV1 and plasma levels of nitrite in asthmatic patients. Avicenna. J. Phytomed. 2017;7(6):531-41.

Webb RC. Smooth muscle contraction and relaxation. Adv. Physiol. Educ. 2003;27(1-4):201-6.

Kim HR, Appel S, Vetterkind S, Gangopadhyay SS, Morgan KG. Smooth muscle signalling pathways in health and disease. J. Cell. Mol. Med. 2008;12(6A):2165-80.

Webb RC. Smooth muscle contraction and relaxation. Adv. Physiol. Educ. 2003;27(1-4):201-6.

Chitaley K, Weber D, Webb RC. RhoA/Rho-kinase, vascular changes, and hypertension. Curr. Hypertension. Rep. 2001;3(2):139-44.

Yuan W, López-Bernal A. Cyclic AMP signalling pathways in the regulation of uterine relaxation. BMC. Pregnancy. Childbirth. 2007;7(1):S10.

Lai PF, Tribe RM, Johnson MR. Differential impact of acute and prolonged cAMP agonist exposure on protein kinase A activation and human myometrium contractile activity. J. Physiol. 2016;594(21):6369-93.

Wehbe N, Nasser SA, Al-Dhaheri Y, Iratni R, Bitto A, El-Yazbi AF, et al. EPAC in vascular smooth muscle cells. Int. J. Mol. Sci. 2020;21(14):5160.

Tanaka Y, Horinouchi T, Koike K. New insights into beta-adrenoceptors in smooth muscle: distribution of receptor subtypes and molecular mechanisms triggering muscle relaxation. Clin. Exp. Pharmacol. Physiol. 2005;32(7):503-14.

Zhao Y, Vanhoutte PM, Leung SW. Vascular nitric oxide: Beyond eNos. J. Pharmacol. Sci. 2015;129(2):83-94.

Warner TD, Mitchell JA, Sheng H, Murad F. Effects of cyclic GMP on smooth muscle relaxation. Adv. Pharmacol. 1994;26:171-94.

Matsuda NM, Miller SM. Non-adrenergic non-cholinergic inhibition of gastrointestinal smooth muscle and its intracellular mechanism(s). Fundam. Clin. Pharmacol. 2010;24(3):261-8.

Jensen KB, Dyrud OK. The smooth muscle relaxing effect of thyme (Thymus vulgaris L). Acta. Pharmacol. Toxicol. (Copenh). 1962;19:345-55.

Van den Broucke CO, Lemli JA. Antispasmodic activity of Origanum compactum. Part 2: Antagonistic effect of thymol and carvacrol. Planta. Med. 1982;45(7):188-90.

Astudillo A, Hong E, Bye R, Navarrete A. Antispasmodic activity of extracts and compounds of Acalypha phleoides Cav. Front. Phytother. Res. 2004;18(2):102-6.

Babaei M, Abarghoei ME, Ansari R, Vafaei AA, Taherian AA, Akhavan MM, et al., Antispasmodic effect of hydroalcoholic extract of Thymus vulgaris on the guinea-pig ileum. Nat. Prod. Res. 2008;22(13):1143-50.

Begrow F, Engelbertz J, Feistel B, Lehnfeld R, Bauer K, Verspohl EJ. Impact of Thymol in thyme extracts on their antispasmodic action and ciliary clearance. Plant. Med. 2010;76(4):311-8.

Micucci M, Protti M, Aldini R, Frosini M, Corazza I, Marzetti C, et al. Thymus vulgaris L. Essential Oil Solid Formulation: Chemical profile and spasmolytic and antimicrobial effects. Biomolecules. 2020;10(6):860.

Meister A, Bernhardt G, Christoffel V, Buschauer A. Antispasmodic activity of Thymus vulgaris extract on the isolated guinea-pig trachea: discrimination between drug and ethanol effects. Plant. Med. 1999;65(6):512-6.

Premrov BB, Prem L, Vake T, Znidarsic N, Snoj T. The effect of thymol on acetylcholine-induced contractions of the rat ileum and uterus under ex vivo conditions. Front. Pharmacol. 2022;13:990654.

How to Cite
Pérez-Sánchez, A. (2023). Relaxant effect of thymol on smooth muscle. Mexican Journal of Medical Research ICSA, 11(22), 23-29.