Papain Gel as a Deproteinizing Agent for Tooth Enamel

Keywords: Papain, Deproteinization, Acid Etching, Tooth Enamel, Acid Etching Pattern

Abstract

Papain gel has been shown to be an effective deproteinizing agent for tooth enamel in several studies. Papain, a proteolytic enzyme derived from papaya, has the ability to break down proteins and remove organic substances adhering to the surface of the enamel. Its application prior to enamel etching has demonstrated significant benefits, such as removing organic residues and improving the adhesion of dental materials. In addition, papain has been shown to be safe and biocompatible in its dental use. Its mechanism of action is based on its ability to break protein bonds, allowing a deep and effective cleaning of the enamel without compromising its structure. Therefore, this study aims to highlight the importance of papain as an effective and safe alternative in dental treatment, emphasizing its ability to improve the adhesion of dental materials and its specific mechanism of action in the removal of organic residues from tooth enamel.

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References

Agarwal RM, Yeluri R, Singh C, Munshi AK. Enamel Deproteinization using Papacarie and 10% Papain Gel on Shear Bond Strength of Orthodontic Brackets Before and After Acid Etching. J Clin Pediatr Dent. 2015;39(4):348-57.

Panchal S, Ansari A, Jain AK, Garg Y. Effects of different deproteinizing agents on topographic features of enamel and shear bond strength - An in vitro study. J Orthodont Sci. 2019;8:17.

Christopher A, Krishnakumar R, Reddy NV, Rohini G. Effect of Enamel Deproteinization in Primary Teeth. J Clin Pediatr Dent. 2018;42(1):45-49.

Bajraktarova-Valjakova E, Grozdanov A, Guguvcevski L, Korunoska-Stevkovska V, Kapusevska B, Gigovski N, et al. Acid Etching as Surface Treatment Method for Luting of Glass-Ceramic Restorations, part 1: Acids, Application Protocol and Etching Effectiveness. Maced J Med Sci. 2018;6(3):568-573.

. Ahmed AM, Nagy D, Elkateb MA. Etching Patterns of Sodium Hypochlorite Pretreated Hypocalcified Amelogenesis Imperfecta Primary Molars: SEM Study. J Clin Pediatr Dent. 2019;43(4):257-262.

Alshaikh KH, Hamama HHH, Mahmoud SH. Effect of smear layer deproteinization on bonding of self-etch adhesives to dentin: a systematic review and meta-analysis. Restor Dent Endod. 2018;43(2):e14.

Justus R, Cubero T, Ondarza R, Morales F. A New Technique With Sodium Hypochlorite to Increase Bracket Shear Bond Strength of Fluoride-releasing Resin-modified Glass Ionomer Cements: Comparing Shear Bond Strength of Two Adhesive Systems With Enamel Surface Deproteinization Before Etching. J Seminars in orthodontics. 2010;16(1), 66–75.

Pithon MM, Ferraz Cde S, de Oliveira Gdo C, Pereira TB, Oliveira DD, de Souza RA,et al. Effect of 10% papain gel on enamel deproteinization before bonding procedure. Angle Orthod. 2012;82(3):541-5.

Ekambaram M, Anthonappa RP, Govindool SR, Yiu CKY. Comparison of deproteinization agents on bonding to developmentally hypomineralized enamel. J Dent. 2017;67:94-101.

Tacias-Pascacio VG, Morellon-Sterling R, Castañeda-Valbuena D, Berenguer-Murcia Á, Kamli MR, Tavano O, et al. Immobilization of papain: A review. Int J Biol Macromol. 2021;188:94-113.

Hobson RS, Rugg-Gunn AJ, Booth TA. Acid-etch patterns on the buccal surface of human permanent teeth. Arch Oral Biol. 2002;47(5):407-12.

Gardner A, Hobson R. Variations in acid-etch patterns with different acids and etch times. Am J Orthod Dentofacial Orthop. 2001;120(1):64-7.

Hobson RS, McCabe JF, Rugg-Gunn AJ. The relationship between acid-etch patterns and bond survival in vivo. Am J Orthod Dentofacial Orthop. 2002;121(5):502-9.

Brauchli L, Steineck M. Etching Patterns of Self-Etching Primers in Relation to Shear Bond Strength on Unground Enamel Samples. Dent J (Basel). 2021;9(11):138.

Bhoomika Ahuja; Ramakrishna Yeluri; M Sudhindra Baliga; AK Munshi. Enamel Deproteinization before Acid Etching – A Scanning Electron Microscopic Observation. J Clin Pediatr Dent. 2010;35(2):169-172.

Silverstone LM, Saxton CA, Dogon IL, Fejerskov O. Variation in the pattern of acid etching of human dental enamel examined by scanning electron microscopy. Caries Res. 1975;9(5):373-87.

Gasmi Benahmed A, Gasmi A, Menzel A, Hrynovets I, Chirumbolo S, Shanaida M, Lysiuk R, Shanaida Y, Dadar M, Bjørklund G. A review on natural teeth whitening. J Oral Biosci. 2022 Mar;64(1):49-58.

Valencia R, Espinosa R, Borovoy N, Pérez S, Ceja I, Saadia M. Deproteinization Effectiveness on Occlusal Enamel Surfaces and Resultant Acid Etching Patterns: An in vitro Study. J Clin Pediatr Dent. 2018;42(6):434-441

Kusumasari C, Abdou A, Nakajima M, Tagami J. Deproteinization of caries-affected dentin with chemo-mechanical caries removal agents and its effect on dentin bonding with self-etch adhesives. J Dent. 2021;109:103665

Bianchi J, Rodrigues Filho LE, César PF, Beck H, Gonzaga CC. Effect of NaOCl on tensile bond strength of dental adhesives. J Dent Res (IADR Abstracts) 2000;79:194

Espinosa R, Valencia R, Uribe M, Ceja I, Cruz J, Saadia M. Resin replica in enamel deproteinization and its effect on acid etching. J Clin Pediatr Dent. 2010;35(1):47-51.

Espinosa R., Valencia R., Rabelero M., Ceja I. Resistencia al desprendimiento de la resina al esmalte desproteinizado y grabado; Estudio de microtensión. J of dental surgery and biomaterials. 2014;3(2):1-6.

Bastos LA, Silva FL, Thomé JPQ, Arnez MFM, Faccioli LH, Paula-Silva FWG. Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells. Braz Dent J. 2019;30(5):484-490.

Pithon MM, Campos MS, Coqueiro Rda S. Effect of bromelain and papain gel on enamel deproteinisation before orthodontic bracket bonding. Aust Orthod J. 2016;32(1):23-30.

Rivera-Botonares R, Oliva-Cruz S, Tineo D, Extraction and purification of papain obtained from three native species of the genus Vasconcellea, Revista de Investigaciones Altoandinas – J of High Andean Research. 2023;25(2),109-116.

Flores J. Proteolytic Activity Of The Papaya Extracted From The Papaya (Carica PAPAYA) Common Variety In The Softening Of Sajino Meat (Tayassu tajacu). J instituonal scientific Tzhoecoen. 2018;10(4),610-629.

Published
2024-08-13
How to Cite
Quintana Sánchez, M. M. (2024). Papain Gel as a Deproteinizing Agent for Tooth Enamel. Mexican Journal of Medical Research ICSA, 13(25). Retrieved from https://repository.uaeh.edu.mx/revistas/index.php/MJMR/article/view/12399