Staining criteria, collection and number of cells evaluated to identify micronuclei in buccal mucosa cells of agricultural workers exposed to pesticides.

Keywords: Micronucleus, biomarker, genotoxic damage, buccal mucosa cells, pesticides

Abstract

Although there are several publications that refer to the basic criteria established for the micronucleus (MN) test in exfoliated buccal cells, there is still a difference in the quantity, method of obtention and staining of the cells for the evaluation of the presence of micronuclei. Objective. Identify the criteria for evaluation of MN in oral mucosa cells exposed to pesticide in investigations carried out. Material and methods. A systematic review was carried out on the internet, based on articles published in Crossref, JCR, Scopus, PubMed, Google academic, using keywords such as: micronuclei, buccal mucosa cells, genotoxic damage, pesticides and biomarker. Results. In the six selected articles, four presented statistically significant values ​​with the presence of MN when comparing the exposed groups with respect to the control groups, and the criteria for staining, collection and number of cells evaluated to identify micronuclei were very varied. Conclusions. It is important to follow validated and standardized protocols for the MN oral cytoma assay. Being considered in all the parameters suggested in the protocol will increase the reliability of the studies and will give the possibility of comparing the results obtained.

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References

Stich HF, Curtis SR, Parida BB. Application of the micronucleus test to exfoliated cells of high cancer risk groups: tobacco chewers. Int. J. Cancer. 1982;30(5):553–9.

Stich HF, Stich W, Parida BB. Elevated frequency of micronucleated cells in the buccal mucosa of individuals at high risk for oral cancer: betel quid chewers. Cancer Lett. 1982;17(2):125–34.

Stich HF, Rosin MP. Quantitating the synergistic effect of smoking and alcohol consumption with the micronucleus test on human buccal mucosa cells. Int. J. Cancer. 1983;31(3):305–8.

Zaridze DG, Blettner M, Matiakin EG, Poljakov BP, Stich HF, Rosin MP, Hoffmann D, Brunnemann KD. The effect of nass use and smoking on the risk of oral leukoplakia. Cancer Detect. Prev. 1986;9(5-6):435–40.

Stich HF, Rosin MP, Brunnemann KD. Oral lesions, genotoxicity and nitrosamines in betel quid chewers with no obvious increase in oral cancer risk. Cancer Lett. 1986;31(1):15–25.

Stich HF, Rosin MP, Hornby AP, Mathew B, Sankaranarayanan R, Nair MK. Remission of oral leukoplakias and micronuclei in tobacco/betel quid chewers treated with beta-carotene and with beta-carotene plus vitamin A. Int. J. Cancer. 1988;42(2):195–99.

Stich HF, Rosin MP. Micronuclei in exfoliated human cells as a tool for studies in cancer risk and cancer intervention. Cancer Lett. 1984;22(3):241–53.

Stich HF, Stich W, Rosin MP. The micronucleus test on exfoliated human cells, Basic Life Sci. 1985;34:337–42.

Stich HF, Dunn BP, DNA adducts, micronuclei and leukoplakias as intermediate endpoints in intervention trials, IARC Sci. Publ. 1988;(89):137–45.

Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps. Mutat Res 2008;659:93-108.

Stich HF, Stich W, Rosin MP, Vallejera MO, Use of the micronucleus test to monitor the effect of vitamin A, beta-carotene and canthaxanthin on the buccal mucosa of betel nut/tobacco chewers. Int. J. Cancer. 1984;34(6): 745–50.

Stich HF, Rosin MP, Vallejera MO, Reduction with vitamin A and betacarotene administration of proportion of micronucleated buccal mucosal cells in Asian betal nut and tobacco chewers, Lancet. 1984;1: 1204–06.

Heddle SM, Short-term JA, Tests for chemical carcinogens, in: S.R.H.F. Stich (Ed.), The Micronucleus Assay. I. In Vivo, Springer-Verlag, Berlin/New York. 1981; 243–49.

Tolbert PE, Shy CM, Allen JW, Micronuclei and other nuclear anomalies in buccal smears: methods development, Mutat. Res. 1992; 271: 69–77.

Tolbert PE, Shy CM, Allen JW, Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users, Am. J. Epidemiol. 1991;134: 840–50.

Thomas P, Holland N, Bolognesi C, et al. Buccal micronucleus cytome assay. Nat Protoc 4(6): 825-37.

Hutter HP, Khan AW, Lemmerer K, Wallner P, Kundi M, Moshammer H. Cytotoxic and Genotoxic Effects of Pesticide Exposure in Male Coffee Farmworkers of the Jarabacoa Region, Dominican Republic Int. J. Environ. Res. Public Health. 2018;15(8) pii: E1641.

Bortoli GM, Azevedo MB, Silva LB. Cytogenetic biomonitoring of Brazilian workers exposed to pesticides: Micronucleus analysis in buccal epithelial cells of soybean growers. Mutat Res. 2009;675(1-2):1–4

Pastor S, Gutiérrez S, Creus A, Cebulska-Wasilewskab A, Marcos R, Micronuclei in peripheral blood lymphocytes and buccal epithelial cells of Polish farmers exposed to pesticides. Mutat Res. 2001;495(1-2): 147–56.

Carbajal-López Y, Gómez-Arroyo S, Villalobos-Pietrini R, Calderón-Segura ME, Martínez-Arroyo A. Biomonitoring of agricultural workers exposed to pesticide mixtures in Guerrero state, Mexico, with comet assay and micronucleus test. Environ Sci Pollut Res Int. 2015;23(3):2513-20.

Cobanoglu H, Coskun M, Coskun M, Çayir A. Results of buccal micronucleus cytome assay in pesticide-exposed and non-exposed group. Environ Sci Pollut Res Int. 2019;26(19):19676-83.

Arévalo-Jaramillo P, Idrobo A, Salcedo L, Cabrera A, Vintimilla A, Carrión M, et al. Biochemical and genotoxic effects in women exposed to pesticides in Southern Ecuador. Environ Sci Pollut Res Int. 2019; 26(24):24911–21.

Ceppi M, Biasotti B, Fenech M, Bonassi S, Human population studies with the exfoliated buccal micronucleus assay: statistical and epidemiological issues, Mutat. Res. 2010;705(1): 11–9.

Bolognesi C, Genotoxicity of pesticides: a reviewof human biomonitoring studies, Mutat. Res. 2003;543 251–72.

McCauley LA, Anger WK, Keifer M, Langley R, Robson MG, Rohlman D, Studying health outcomes in farmworker populations exposed to pesticides, Environ. Health Perspect. 2006;114(6) 953–60.

Torres-Bugarín O, Zavala-Cerna MG, Nava A, Flores-García A, Ramos-Ibarra ML. Potential uses, limitations, and basic procedures of micronuclei and nuclear abnormalities in buccal cells. Dis Markers. 2014;2014: 956835.

Published
2021-07-05
How to Cite
Santiago-Manzano , D. (2021). Staining criteria, collection and number of cells evaluated to identify micronuclei in buccal mucosa cells of agricultural workers exposed to pesticides . Mexican Journal of Medical Research ICSA, 9(18), 34-38. https://doi.org/10.29057/mjmr.v9i18.5717