Evaluation of the cytotoxic activity of microalgal chlorophyll derivatives in cell lines
Abstract
Environmental, genetic factors as well as bad habits of contemporary life have deteriorated their quality in the population, increasing chronic degenerative diseases such as heart disease, Diabetes mellitus or cancer. In the case of cancer, surgery or chemotherapy drastically affect the quality of life of the patient. Recent researches seek harmless substances for treatment such as phototherapy, which is based on the administration of oxo chlorophyll as a derivative of the pigment, which is absorbed by cells with high cell division, such as cancer cells, and activated by exposing the cells that accumulated it to red light causing the formation of free radicals that alter cellular metabolism. Therefore, chlorophyll A was obtained from Chlorella spp. in photobioreactors with modified Bold basal medium, using rice starch as carbon source in 12 h photoperiods. Chlorophyll A was purified by silica gel column chromatography eluting with absolute ethanol and it was concentrated by rotary evaporator. Oxychlorophyll A was obtained by pyrolysis under anoxic conditions and by acidification. Its antioxidant activity was measured by the guaiac method. Its cytotoxic effect in Artemia salina was determined at 1:13, 1:20 and 1:40 dilutions of oxochlorophyll A. Higer cytotoxicity was registered than that obtained by pyrolysis. LD50 calculated (Graphpad Prism) was 1.198%. Future work will evaluate its cytotoxic effect in healthy and malignant human cell lines and in mice.
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References
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