In vitro evaluation of hemocompatibility in hydrothermally synthesized HA
Abstract
Hydroxyapatite (HA) is a biomaterial to restore bone tissue due to its biocompatibility. Among the biological tests to be performed to evaluate it is the hemocompatibility test. In this study, HA particles were synthesized by the hydrothermal method to determine its hemocompatibility. The size was controlled by the use of surfactants, a 22 factorial experimental design was performed, using the following surfactants: EDTA (1 and 2mM) and Pluronic F-127 (3 and 6 mM). The presence of the hexagonal phase was validated by X-ray diffraction and Fourier transform infrared spectroscopy. The use of surfactant promotes the change in morphology of hydroxyapatite, rods were obtained for the use of EDTA and needles and plates were obtained for the use of Pluronic F-127. Hemocompatibility was determined by hemolysis test. Most of the HA particles synthesized using Pluronic F-127 and EDTA show less than 5% hemolysis suggesting that they are highly compatible according to ISO 10993-4, and could be used in biomedical applications.
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