Criterios de tinción, recolección y número de células evaluadas para identificar micronúcleos en células de mucosa bucal de trabajadores agrícolas expuestos a pesticidas
Resumen
Aunque existen varias publicaciones que refieren los criterios básicos establecidos para la prueba de micronúcleos (MN) en células bucales exfoliadas aún hay diferencia en la cantidad, método de obtención y tinción de las células para la evaluación de presencia de micronúcleos. Objetivo. Identificar los criterios para evaluación de MN en células de mucosa oral de expuestos a pesticidas en investigaciones realizadas. Material y métodos. Se realizó una revisión sistemática en la red de internet, con base en artículos publicados en crossreff, JCR, Scopus, PubMed, Google académico, mediante palabras clave como: micronúcleos, células de mucosa bucal, daño genotóxico, pesticidas y biomarcador. Resultados. En los seis artículos seleccionados cuatro presentan valores estadísticamente significativos con presencia de MN al comparar los grupos expuestos respecto a los grupos control, y los criterios de tinción, recolección y número de células evaluadas para identificar micronúcleos son muy variados, Conclusiones. Es importante seguir los protocolos validados y estandarizados para el ensayo de MN citoma bucal. Al ser considerado en todos los parámetros sugeridos en el protocolo aumentará la fiabilidad de los estudios y dará la posibilidad de comparar los resultados obtenidos.
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