Encapsulación de ficocianina en una matriz polimérica de quitosano-TPP por gelación ionotrópica

María Elena García-Callejas; Sharon Zurisadai Meza-Herrera; Johan Uriel García-Pérez; Mónica Araceli Camacho-González; Nikte Yoliztli Martinez-Palma

doi:10.29057/icbi.v11iEspecial5.11826

María Elena García-Callejas Universidad Tecnológica de Tecámac https://orcid.org/0009-0008-6249-3900
Sharon Zurisadai Meza-Herrera Universidad Tecnológica de Tecámac https://orcid.org/0009-0000-7804-0801
Johan Uriel García-Pérez Centro de Investigación e Innovación Tecnológica-IPN https://orcid.org/0009-0002-8966-5318
Mónica Araceli Camacho-González Universidad Tecnológica de Tecámac https://orcid.org/0000-0001-5890-6313
Nikte Yoliztli Martinez-Palma Universidad Tecnológica de Tecámac https://orcid.org/0000-0002-1313-6842

DOI: https://doi.org/10.29057/icbi.v11iEspecial5.11826

Keywords: ionotropic gelation, chitosan, nanoparticles, phycocyanin

Abstract

Phycocyanin is a protein present in Spirulina, it has different biological activities such as antioxidant, anti-inflammatory, antitumor and immunomodulatory capacity that make it a biocompound with potential to treat diseases, however, it presents stability and availability problems when administered in biological systems. In this project, it was proposed to encapsulate phycocyanin in a chitosan polymer matrix to protect it from pH, temperature and physiological conditions that compromise its biological activity. The ionotropic gelation technique and tripolyphosphate (TPP) were used as a cross-linking agent. Different ratios of chitosan and TPP, as well as protein, were tested. The best results were obtained with the 9:1 ratio and 10% protein concentration, obtaining 12.6 and 33.21% charge and association efficiency respectively. Through FTIR spectroscopy, the interaction between the amino groups of chitosan and phosphate could be verified. of the TPP, the particle solution presented a surface charge of -37.3 mV and a polydispersity percentage of 84.4.

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