Quantification of the urinary elimination of acetylsalicylic acid in humans

Abstract

Salicylate acetic acid (ASA), is rapidly absorbed by the stomach and the upper part of the intestine, the maximum level in the salicylate plasma is between 60 and 90 minutes. After absorption as ASA, it is hydroxylated to acetic acid and salicylate by esterases in the tissues and blood, the effects of ASA on the cell are complex and the main mechanisms include the inhibition of the enzyme oxygen cycle and the concomitant blockade of both prostaglandins and synthesis of thromboxane. This paper aims to determine the elimination of ASA in the urine of healthy volunteers and the influence of the presentation of the drug and the gastrointestinal pH in its elimination.  To meet the objective, six healthy volunteers were required to take 250mg of ASA in the two presentations of the drug accompanied by different drinks (juice of one, orange, efferent, milk, mango yogurt), a spectrophotometer was used in the samples of urine collected at different times after the administration of the drug and the salicylate content in each sample was determined, measuring the PH and the volume collected from each urine sample, A 2 ml aliquot of the urine sample was taken, then 8 ml of 0.1N hydrochloric acid was added, stirred and filtered, 5 ml of the filtrate and 1 ml of the trinder relative were also taken. resulting in a variation in the volume of the urine and in the pH according to the pharmaceutical form and the previously ingested beverage. After carrying out the development of the practice in a successful way we were able to analyze and verify the results obtained, the purification of ASA could be expressed in ml / mm and thus verify the importance of the presentation of the drugs in the pharmacokinetics and the influence of Gastrointestinal ph in healthy patients, concluding that the concentrations obtained from salicylate in each collection interval were normalized.