Hemp hydroponics to obtain CBD for therapeutic purposes
Abstract
Cannabidiol or CBD is a non-psychoactive cannabinoid derived from fiber-type Cannabis (hemp), used as a therapeutic alternative for the treatment of various pathologies. Derived from the medical importance of CBD, and its pharmacological demand, the need arises to homogenize the process of obtaining CBD extracts from hemp cultivation. A hydroponic system makes it easier to determine conditions to produce this plant species. The most important advantages of hydroponics are that the soil is not required as a support or source of nutrients, pest control, adaptation to any space and climatic condition, and it would even increase yields. Therefore, a proposal for a hydroponic hemp cultivation system to obtain CBD is presented; includes system layout, substrates, temperature, containers, lighting, aeration, humidity, pH, drainage and irrigation system, nutrient solution, and suggested electroconductivity. In addition, a bibliographic review of the identification of CBD by HPLC is included.
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Ángeles, G.E., Brindis, F., Cristians, S., Ventura, R. (2014).Cannabis sativa L., una planta singular. Revista Mexicana de ciencias farmacéuticas 45 (4), 1-6.
Lars, A., Franziska, P., Alain, B., Stefan, K., Wolfgang, W.*, Werner, B. (2014). Simultaneous quantification of delta-9-THC, THC-acid A, CBN and CBD in seized drugs using HPLC-DAD. Forensic Science International 24, 107–111. DOI: 10.1016/j.forsciint.2014.06.008
Attard, M., Bainier C., Reinaud, M., Lanot, A., McQueen-Mason, J., Hunt, J. (2018). Utilisation of supercritical fluids for the effective extraction of waxes and Cannabidiol (CBD) from hemp wastes. Industrial Crops & Products 112, 38–46. DOI: 10.1016/j.indcrop.2017.10.045
Cervantes, J. (2007). Marihuana: Horticultura del Cannabis la Biblia del Cultivador Medico de Interior y Exterior. Vol.1. Van Patten Publishing, Estados Unidos, Ch. 6-13, pp. 106-307.
Citti, D., Braghiroli, A., Vandelli, G. (2018). Cannazza, Pharmaceutical andbiomedical analysis of cannabinoids: a critical review. Journal of Pharmaceutical and Biomedical Analysis 147, 565–579. DOI: http://dx.doi.org/10.1016/j.jpba.2017.06.003.
Da Porto, C. Natolino, A., Decorti, D. (2015). Effect of Ultrasound Pre-Treatment of Hemp (Cannabis sativa L.) Seed on Supercritical CO2 Extraction of Oil. Association of Food Scientists & Technologists 52, 1748–1753. DOI: 10.1007/s13197-013-1143-3
De Backer, B., Debrus, B., Lebrun, P., Theunis, L., Dubois, N., Decock, L., Verstraete, A., Hubert, P., Charlier C. (2009a) Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material. Revista de cromatografía 32, 4115–4124.
Elango D., Dony K., Kumar H., Rajendran, K., Thoomatti, V.K., Dharmaraj D., Vadakankoor, C., Misericordia, W., Mishra, R., Vembu, K., Wang, X. (2022b). Intervenciones agronómicas, de mejoramiento y biotecnológicas para mitigar los problemas de toxicidad de metales pesados en la agricultura. Journal of Agriculture and Food Research 10, 1-8.
Farag, S. & Kayser, O. (2017). The Cannabis Plant: Botanical Aspects. Manual de Cannabis y Patologías Relacionadas 1, 1-12. DOI: 10.1016/B978-0-12-800756-3.00001-6
Ferioli, V., Rustichelli, C., Pavesi, G., Gamberini, G. (2000). Analytical characterisation of Hashish samples. Chromatographia 52, 39–44. DOI: 10.1007/BF02490790
FDA. (2020, 01 de octubre). Regulación de la FDA sobre el Cannabis y los productos derivados del Cannabis, incluido el cannabidiol (CBD). Razón Pública. https://www.fda.gov/news-events/public-health-focus/fda-regulation-cannabis-and-cannabis-derived-products-including-cannabidiol-cbd
Hädener, M., König, S., Weinmann, W. (2019). Quantitative determination of CBD and THC and their acid precursors in confiscated cannabis samples by HPLC-DAD. Forensic Science International 1, 142-150. DOI: 10.1016/j.forsciint.2019.03.046
Hazekamp, A. (2006). An evaluation of the quality of medicinal grade cannabis in the Netherlands. Cannabinoids 1(1):1–9
Kaul, N., Kreml, R., Austria, J.A., Richard, M.N., Edel, A.L., Dibrov, E., Hirono, S.M.D., Zettler, M.E., Pierce, G.N. (2008). A Comparison of Fish Oil, Flaxseed Oil and Hempseed Oil Supplementation on Selected Parameters of Cardiovascular Health in Healthy Volunteers. Journal of the American College of Nutrition 27:1, 51-58, DOI: 10.1080/07315724.2008.10719674
Landa L., Jurica J., Sliva J., Pechackova M., Demlova R. (2018). Medical cannabis in the treatment of cancer pain and spastic conditions and options of drug delivery in clinical practice. Biomedical Papers 28(3), 19-20. DOI: 10.5507/bp.2018.007
Mudge, E.M., Murch, S.J., Brown, P.N. (2017). Leaner and greener analysis of cannabinoids. Bioanal Chem 1, 3153–3163. DOI: 10.1007/s00216-017-0256-3
Pellegrino, C., Buonerba, C., Cannazza, G., D'Auria, J., Ermete, Fulgione A., Di Stasio A., Pierri B., & Gallo A. (2021). Una revisión del cáñamo como alimento y suplemento nutricional. Cannabis and Cannabinoid Research 6, 19-27. DOI: 10.1089/can.2020.0001
Rawson, M.H., Gómez, M.H. (2001). Trigo Regado. Manejo del cultivo. Organización de las Naciones Unidas para la Agricultura y la Alimentación. FAO. Roma.
Raharjo, T.J, Verpoorte R. (2001). Methods for the analysis of cannabinoids inbiological materials: a review. Phytochemical analysis 15, 79–94. DOI: 10.1002/pca.753
Rivera, V.M., Parra, M.C. (2016). Cannabis: efectos en el sistema nervioso central. Consecuencias terapéuticas, sociales y legales. Revista Médica del Instituto Mexicano del Seguro Social 54(5), 629-630.
Rustichelli, C., Ferioli, V., Vezzalini, F., Gamberini. G. (1996a). Simultaneous separation and identification of hashish constituents by coupled liquid chromatography mass spectrometry (HPLCMS). Chromatographia 43, 129–134.
Rustichelli, C., Ferioli, V., Baraldi, M., Zanoli, P., Gamberini, G. (1998b). Analysis of cannabinoids in fiber hemp plant varieties (Cannabis sativa L.) by high performance liquid chromatography. Chromatographia 8(7), 215–222.
Swift, W.A. Wong, Li, K.M., Arnold, J.C., McGregor, I.S. (2013). Analysis of cannabisseizures in NSW, Australia: cannabis potency and cannabinoid profile. PLoS One 8 (7),1-9. DOI: doi.org/10.1371/journal.pone.0070052
Turner C.E., Cheng P. C., Lewis G. S., Russell M. H., Sharma G. K. (1979). Planta médica, 37(11): 217-221.
UNODC. (2010). Métodos recomendados para la identificación y el análisis del cannabis y los productos del cannabis. United Nations Publication. New York.
